The service
In the Single Cell Analysis service, we use advanced cytometry techniques, combining conventional cytometers and a spectral cytometer with a sorting module. Flow cytometry is a technique that allows the simultaneous measurement of multiple optical characteristics (light scattering and fluorescence) of each of the cells or particles present in a suspension. This allows us to define the properties of a cell population or its subpopulations. Cytometers have a combined flow, optical and electronic system to carry out this process. The flow system introduces and restricts the cells for individual analysis, the optical system excites the sample and collects the light signals coming from the sample, and the electronic system converts the optical signal into an electronic signal and digitises it for computer analysis.
Current research studies in the field of cell biology investigate the individual properties of the cells that make up a system. To do this, it is necessary to be able to purify cell populations in order to remove them from a sample or to enrich it. Cell sorting by flow cytometry or cell sorting, carried out by separation of electrically charged droplets, is the process of physically separating cell populations that differ in one or more parameters that are analysable by flow cytometry. This process is essential in investigations of complex biological processes, such as immune response and disease progression. Our unit is designed to facilitate innovative discoveries through these cutting-edge technologies.
Conventional cytometers offer highly resolved analyses, but are limited in the number of fluorochromes they can detect simultaneously and require offsets, one of the most complex elements in the use of cytometry. In contrast, spectral cytometry allows potentially more than 25 fluorochromes to be discriminated in a single marker tube, without requiring compensation between the different fluorochromes. It also allows the use of very similar fluorescent molecules as they can be distinguished from each other due to the unique spectral fingerprint that allows them to be differentiated.
Highlights
- Advice on suitable panels and monoclonal antibodies on a customised basis for each investigation
- Portfolio of conventional and spectral flow cytometry services
- Possibility of isolating and collecting specific cells based on their unique characteristics, facilitating more precise and personalised subsequent studies in collaboration with the IRYCIS Central Support Units.
