• Microarray printing robot (SpotArray 72, PerkinElmer)
• Reading scanner (ScaArray Express, PerkinElmer)
• Liquid handling robot (Janus Varispan, PerkinElmer)
• Semiautomatic hybridizer (BioMixer ™ II, CapitalBio)
• Real-time thermal cycler (LightCycler 480, Roche)
• Computer support for microarray analysis
• Three freezers connected to a radio control alarm system

Personalised microarray design

** Consult prices with the unit

Preparation of samples

• Total RNA extraction (Tripure) - 1 sample
• Total RNA extraction (Columns) - 1 sample
• mRNA extraction (Columns) - 1 sample
• RNA analysis and quantification (NanoDrop and integrity gel) - 8 samples

Microarrays

• DNA and protein microarray printing *
• Retrotranscription and RNA labeling (two-color labeling with Cy3 or Cy5 without amplification)
• Hybridization (DNA or proteins) **
• Microarray reading (DNA or proteins)

Real-time PCR (LightCycler 480 SYBR green)

• Probe design (for SYBR green)
• RNA retranscription
• Real-time PCR (by gene and by triplicate sample)

* Price of printing or crystals is not included

** Price of the microarray is not included