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Microscopy, histology and comparative pathology

Leader

Ignacio Ruz Caracuel

Personnel

Silvia Sacristán (Histología)

Begoña Cuevas (Microscopía)

Contact

Responsable Unidad: ignacio.ruz(ELIMINAR)@salud.madrid.org

Consulta y solicitudes: uca.histologia.irycis(ELIMINAR)@gmail.com

  • Equipment
    • CryoStar NX50 cryostat for frozen tissue samples.
    • Microm microtome model HM325 for paraffin-embedded tissue samples.
    • HybEZ II ACD hybridization oven.
    • Tissue microarrayers.
    • Extractor hood
    • Nikon ECLIPSE Ti confocal microscope with image acquisition system.
    • Zeiss Axio Observer 7 fluorescence inverted microscope.
    • ECLIPSE Ci fluorescence microscope equipped with Nikon DS-Ri1 camera.
    • Motic BA310 optical microscope equipped with MOTICAMS3 camera.
    • Image analysis workstation. NIS ELEMENTS software.
  • Noteworthy
    • Portfolio of services with basic histological techniques (paraffin and frozen tissues), immunohistochemistry, immunofluorescence, aptahistochemistry, TUNEL and mRNA morphological detection systems.
    • Optical and fluorescence microscopy equipment, with confocal microscopy systems and fluorescence study of in vivo samples.
    • Advice on the interpretation of human histological sections and animal models by pathologists.
    • Acquisition of histological images and advice for quantification using free use software (Fiji, QuPath).
  • Services portfolio
    • Preparation and cutting of paraffin blocks
    • Preparation and cutting of frozen blocks
    • Hematoxylin and eosin staining
    • PAS staining
    • Masson's trichrome staining
    • Immunofluorescence
    • Immunohistochemistry
    • Aptahistochemistry
    • TUNEL
    • RNAscope
    • Tissue microarray construction (TMA)
    • Confocal microscopy (Nikon ECLIPSE Ti equipment)
    • In vivo microscopy (Zeiss equipment)
    • Fluorescence microscopy
    • Section scanning
    • Image analysis and comparative pathology
    • Technical support in histology
    • Technical advice to IRYCIS members