Laboratory 1 - Preamplification Zone

• Chemagic Magnetic Separation Module I (Chemagic MSM I) (Chemagen, PerkinElmer)
• Nanodrop 2000 spectrophotometer
• Agilent 2200 TapeStation System bioanalyzer
• Qubit 2.0 fluorometer
• Thermoscientific Varioskan LUX
• Freezers -20 ºC

Laboratory 2 - Post-Amplification Zone

• 2 System 9700 PCRs from AppliedBiosystems
• VeritiPro 96-Well ThermalCycler from AppliedBiosystems
• MiSeq sequencer from Illumina

• Collaboration agreements signed with several technology companies belonging to ASEBIO (Spanish Association of Biocompanies), and with national and international public and private universities.
• Teaching in courses on Translational Research Techniques, Omic Sciences and Massive Sequencing Techniques (NGS).
• Quality assurance in the storage and processing of samples, maintaining their traceability, following standardized work protocols (SOPs). Laboratory and equipment cleaning, maintenance and verification plan.
• Continuous training of laboratory personnel.
• Main computer cluster with 32 cpu-cores and 526Gb of RAM memory.
• 40Tb storage cabinet and a dedicated fiber optic broadband internet connection.
• All IRYCIS IPs can apply for an account on the server free of charge.
• All IRYCIS internal staff accounts include free of charge: 400 core-hours of computing per month and 1Tb of storage. 
• Discounts are not cumulative between different accounts of the same IP. 
• Each IRYCIS IP is entitled to only one account with these discounts, any extra account requested will share the discounts of the main account.

**Rates are available for all services. Please, consult at the Unit

Nucleic acid extraction - DNA*

Service	Unit	Conditions
DNA extraction from blood 2 ml_manual	€/sample or reaction	1 sample
DNA extraction from 250 mg faeces_manual		1 sample
DNA extraction from blood 5 ml_manual_head 12		1 sample
DNA extraction from blood 400 µl_head 96		96 samples
DNA extraction from 2 ml saliva_head 12		Consult price for < 12 samples (1)
DNA extraction from dried blood (paper 3.2 mm)_head 96		Consult price for < 96 samples (1)
DNA extraction from fresh tissue 10 mg (tissue or animal cells)_head 12		Consult price for < 12 samples (1)
DNA extraction from fresh tissue 10 mg (tissue or animal cells)_head 96
DNA extraction from FFPE tissue 10 µm section_head 96		Consult price for < 96 samples (1)
DNA extraction from 1,5 ml bacterial culture_head 96		Consult price for < 96 samples (1)

RNA extraction

Service	Unite	Conditions
RNA extraction from 2,5 ml of blood in PAX-gene tubes_head 12	€/sample or reaction	Consult (1)
Special DNA and RNA extraction
Ask for other fees: DNA from amniotic fluid, plasma, buccal swab, viral DNA/RNA, etc.		Consult (1)

Nucleic acid analysis (1)

Service	Unit	Conditions
High sensitivity DNA analysis by capillary electrophoresis_Tape Station 2200	€/sample or reaction	No. of samples of 48 or more units consult price list (1)
Wide range DNA analysis by capillary electrophoresis_Tape Station 2200
Genomic DNA analysis by capillary electrophoresis_Tape Station 2200
High sensitivity RNA analysis by capillary electrophoresis_Tape Station 2200
Wide range RNA analysis by capillary electrophoresis_Tape Station 2200

Nucleic acid quantification

Service	Unit	Conditions
DNA or RNA quantification by UV absorbance (Nanodrop) and by fluorimetry (Qubit 2.0)	€/sample or reaction
Quantification of DNA or RNA by UV absorbance (Nanodrop)
Quantification of DNA or RNA by UV absorbance (Nanodrop). 96-sample plate.		Minimum no. of samples = 48
Quantification of DNA or RNA by UV absorbance (Nanodrop). Self-service (6)
Quantification of DNA or RNA by fluorimetry_Qubit 2,0
Quantification of DNA or RNA by fluorimetry_Qubit 2,0. 96-sample plate.		Minimum no. of samples = 48
Quantification of DNA or RNA by fluorimetry_Qubit 2.0. Self-service (6)
Primary bioinformatics analysis of sequencing data
Molecular determination in biological fluids

Sanger sequencing

Service	Unit
Sanger sequencing tube	€/sample or reaction
Sanger sequencing plaque (48/96)

NGS library construction

Service
Library construction (2)
Metagenomics (RNAr 16S sequencing)

NGS library construction + MiSeq run (5)

Service
Run Kit v2 PE: 2X150pb (300 cicles): ~30 million reads
Run Kit v3 PE: 2X75pb (150 cicles): ~50 million reads
Run Kit v3 PE: 2X300pb (600 cicles): ~50 million reads
Run Kit v2 Micro PE: 2X150pb (300 cicles): ~8 million reads
Run Kit v2 Micro PE: 2X150pb (300 cicles): ~2 million reads
Run Kit v2 Micro PE: 2X150pb (500 cicles): ~2 million reads
Run in MiSeq (includes cartridge and run reagents) (3) (5)
Run Kit v2 PE: 2X150pb (300 cicles): ~30 million reads
Run Kit v3 PE: 2X75pb (150 cicles): ~50 million reads
Run Kit v3 PE: 2X300pb (600 cicles): ~50 million reads
Run Kit v2 Micro PE: 2X150pb (300 cicles): ~8 million reads
Run Kit v2 Nano PE: 2X150pb (300 cicles): ~2 million reads
Run Kit v2 Nano PE: 2X250pb (500 cicles): ~2 million reads

MiSeq run (equipment use only) (4)

Service
Run 150 cicles
Run 300 cicles
Run 600 cicles

NGS library construction + NextSeq 550 run (5)

Service
Run Kit v2 NextSeq High Output PE: 2X150pb (3000 cicles): >400 million reads
Run Kit v2 NextSeq High Output PE: 2X75pb (150 cicles): >400 million reads
Run Kit v2 NextSeq  Mid output PE: 2X150pb (3000 cicles): >130 million reads
Run Kit v2 NextSeq  Mid output PE: 2X75pb (150 cicles): >130 million reads

NextSeq 550 run (includes cartridge and run reagents) (3) (5)

Service
Run Kit v2 NextSeq High Output PE: 2X150pb (3000 cicles): >400 million reads
Run Kit v2 NextSeq High Output PE: 2X75pb (150 cicles): >400 million reads
Run Kit v2 NextSeq Mid output PE: 2X150pb (3000 cicles): >130 million reads
Run Kit v2 NextSeq Mid output PE: 2X75pb (150 cicles): >130 million reads

NextSeq 550 (equipment use only) (4)

150 cicles run
300 cicles run

NovaSeq 6000 run (1)

Service
Runs from 800 million reads

CGH arrays (comparative genome hybridisation)

Service
CGH 60k array
CGH 180k-HD array
CGH 180k-SNP array

MLPA

Service	Unit	Conditions
MLPA	€/sample or reaction	For several samples, please consult prices (1)

Microsatellite genotyping

Service
Microsatellite analysis

Citogenetics

Service	Unit
Karyotype	€/sample or reaction
FISH

Miscellaneous (1)

Service
Genomic DNA controls
Real-time PCR/Allelic discrimination
Other genetic/genomic determinations/studies

Service

Service	Unit
Use of thermocyclers	€/hour
PCR product purification using ExoSap	€/sample or reaction

Consultancy

Service	Unit
Design of experiments/oligonucleotides	€/hour
Experimental technical advice	€/hour

Bioinformatic analysis (performed by the Bioinformatics Unit) (1)

Service
Secondary and/or tertiary analysis of raw data

NOTES

1. Please consult rates, as the basic cost varies for each application and for each experiment.
2. Re-sequencing of human gene panels (PCRs user, multiplex PCRs, capture), RNAseq, mitochondrial genome, microorganism genomes, influenza A virus, 16S metagenome, aptamers, miRNAs, etc. 
3. Running reagents + flowcell + use of equipment.
4. Use of equipment only; the user provides the cartridge and reagents for the run.
5. According to budget.
6. Self-service includes use of equipment always supervised by Unit staff.